By Eli Grushka, Nelu Grinberg
For greater than 4 many years, scientists and researchers have relied upon the Advances in Chromatography sequence for the main up to date info on a variety of advancements in chromatographic equipment and purposes. protecting the state-of-the-art in separation technology, this quantity keeps to offer well timed, state of the art stories on chromatography within the fields of bio-, analytical, natural, polymer, and pharmaceutical chemistry. Compiled by means of prime researchers from all over the world, new chapters conceal themes with regards to countercurrent chromatography and large-scale genotyping in addition to cyclic voltammetry detection, a robust instrument for opting for the electrochemical features of natural compounds.
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Additional info for Advances in Chromatography, Volume 47
D. packed capillary columns that generated nanoscale flows of 20–400 nL/min. , >80 cm) LC columns , and generate a separation peak capacity of up to ~1000. 2 cm/s (close to the optimal value). The robust use of columns with such small inner diameter has been enabled by implementing online solid-phase extraction (SPE, see below). ) high-resolution capillary LC columns become available, a further reduction of the flow rate to <10 nL/min is practical. However, the response of ESI-MS to <10 nL/min LC mobile-phase flow, and how much more sensitivity can be gained by reducing the mobile-phase flow from 20 to ≤10 nL/min are unknown.
69. Annan, R. , Huddleston, M. , Deshaies, R. , and Carr, S. A. 2001. A multidimensional electrospray MS-based approach to phosphopeptide mapping. Anal. Chem. 73: 393–404. 70. Neubauer, G. and Mann, M. 1999. Mapping of phosphorylation sites of gel-isolated proteins by nanoelectrospray tandem mass spectrometry: Potentials and limitations. Anal. Chem. 71: 235–242. 71. , Liu, Y. , Smith, C. , and Pramanik, B. N. 2004. Mass spectrometric characterization of MEK1 phosphorylation during expression in insect cells.
Natl. Acad. Sci. USA 101: 9528–9533. 44. Mikesh, L. , Coon, J. , Syka, J. , and Hunt, D. F. 2006. The utility of ETD mass spectrometry in proteomic analysis. Biochim. Biophys. Acta 1764: 1811–1822. 45. , Johnson, G. , and Burgess, A. W. 1980. Direct stimulation by purified GM-CSF of the proliferation of multipotential and erythroid precursor cells. Blood 55: 138–147. 46. , Pramanik, B. , Nagabhushan, T. , and Trotta, P. P. 1993. Isolation and characterization of a resistant core peptide of recombinant human granulocyte-macrophage colonystimulating factor (GM-CSF); confirmation of the GM-CSF amino acid sequence by mass spectrometry.
Advances in Chromatography, Volume 47 by Eli Grushka, Nelu Grinberg