By W. W. Christie
This can be the 3rd quantity of an occasional sequence of evaluate volumes facing points of lipid technique. As with the 1st volumes, themes were chosen which were constructing quickly in recent times and feature a few significance to lipid research. The authors are all prime foreign experts.
Topics coated comprise: research of positional isomers of glycerolipids through non-enzymatic tools, separation of phospholipid sessions through high-performance liquid chromatography, and nuclear magnetic resonance spectroscopy and lipid part behaviour and lipid diffusion, between others
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Extra info for Advances in Lipid Methodology. Volume 3
These cross-checks are also important in non-enzymic analyses in order to avoid pitfalls from incomplete resolution of enantiomers, losses of polyunsaturates due to peroxidation during lengthy processing and losses due to insolubility of derivatives, as well as those due to non-representative transmethylation of the fatty acids for GLC. E. BIOLOGICAL SIGNIFICANCE The importance of positional analysis arises from the recognition that natural triacylglycerols are mixtures of enantiomeric and racemic molecular species of non-random molecular association of fatty acids [7,41 ].
Alternatively, the fatty acid composition of the sn-2-position has been derived from the 2-monoacylglycerol released by pancreatic lipase . The results of stereospecific distribution of the fatty acids ADVANCES IN LIPID METHODOLOGY - THREE 31 determined by the separation of the diastereomeric diacylglycerols gave results, which agreed closely to those obtained previously by enzymatic methods , where parallel data were available. The compositions of the fatty acids in the sn-1-, sn-2- and snoftriacylglycerols analysed by chiral-phase separation of the sn-1,2- and sn-2,3-diacylglycerols are also calculated indirectly [30,89,110,112,113].
E. BIOLOGICAL SIGNIFICANCE The importance of positional analysis arises from the recognition that natural triacylglycerols are mixtures of enantiomeric and racemic molecular species of non-random molecular association of fatty acids [7,41 ]. It has been assumed that knowledge of the positional distribution and molecular association of the fatty acids will help to understand the biological function and the chemical and physico-chemical properties of natural fats and oils. Although methods of chromatographic resolution and stereospecific assays of triacylglycerols have been known and applied to positional analyses for some 30 years, the hopes of understanding the physiological significance of the triacylglycerol structure have been slow in realization.
Advances in Lipid Methodology. Volume 3 by W. W. Christie